Theme-based tests provide an assessment tool that instructs as well as provides a single general context for a broad set of biochemical concepts. A single story line connects the questions on the tests and models applications of scientific principles and biochemical knowledge in an extended scenario. Theme-based tests are based on a set of interrelated journal articles or a fictional story derived from popular culture or local events that interweaves biochemical principles into a story line. Theme-based tests engage students during an exam and promote their creativity.