The importance of ICTs has become the undisputed in the present century. Studies have been conducted to investigate the use of ICTs with the goal of increase in quality of teacher education for a long time. This study is a cross-cultural comparison in terms of pre-service teachers' level of ICT usage, ICT knowledge and attitudes. The study was conducted in three different universities, two in Turkey and one in Bosnia and Herzegovina. A total of 709 pre-service teacher participated in the study, 385 from Turkey and from 324 Bosnia and Herzegovina. To this end, three different questionnaires were used: the ICT usage for educational purposes scale (ICT-U), the ICT knowledge scale (ICT-K), and the Internet attitude scale (IAS). According to the results significant differences were found between two countries in terms of ICT usage. ICT knowledge was determined as the most explaining variable for the level of ICT use. Culture is also found to be predictive. The study results revealed the need to take into consideration cultural differences in ensuring the integration of ICTs.