The present study explores LIS students' perceptions toward the assimilation of e-books in the library. The research was conducted during the summer semester of the 2010 academic year in Israel and 144 students participated in the research. The study used three questionnaires: a personal details questionnaire, students' perceptions towards e-books assimilation in the library, and a cognitive appraisal questionnaire. The main findings suggest that LIS students have positive perceptions regarding the assimilation of e-books in the library. They are aware of the change and possible effect it may have on library collections, budget, and policy. Those students who have higher perceptions towards the assimilation of e-books in the library are more challenged by this phenomenon. Older students feel challenged to experience, learn and assimilate e-books more than younger students. This study highlights LIS students' perceptions towards e-books and is relevant for librarians, information scientists and LIS educators, helping them better understand, assimilate and explore the phenomenon of e-books in libraries.