The method for producing monoclonal antibodies (MAbs) was first published in 1975. MAbs have revolutionised research, diagnosis, and disease treatment approaches. While students need a good understanding of MAb production, teaching the key steps via traditional methods can be challenging. Numerous resources exist, but these focus on hybridoma selection omitting other key steps. In 2016, an interactive simulation was developed where students produced a virtual MAb. The simulation was used in conjunction with a lecture and tutorial, providing an alternate approach for students to learn and test their understanding. The simulation is interactive, placing students in charge of all key decisions, with immediate feedback provided at all stages. The impact of the simulation on student learning was assessed between 2017-19. Ten formative multiple-choice questions were included so students could test their understanding. The impact of the simulation on student performance was assessed in a quiz. Students that used the simulation consistently scored significantly higher in all assessment items than students who did not access the simulation. Students were engaged and the positive feedback indicated that they enjoyed this novel, alternative teaching method. This demonstrates that a simulation is a novel, learning aid for teaching students all aspects of MAb production.