This article aims to explore the impact of AR on the learning outcomes of college freshmen and their knowledge about the biology lab course. In a study, a basic biology lab course app was developed using AR technology. The students used this app to prepare for a basic biology lab course, and carry on self-learning without the help of instructors. The results showed that by integrating AR technology into the instruction, the students took on a more positive autonomous learning attitude; they were able to gain a better grasp of the basic biology lab knowledge through the interactive operation as well as cooperative learning. In addition, the students were found to have learned the importance of scientific knowledge with this interactive technology. The basic biology lab course app developed in the study offered the benefits of autonomous learning, situational simulation and interactive experience.