Many plant biologists claim that there is a tremendous bias towards the study of animals in science curricula, however, there is even less attention given to topics on fungi. On average, Biology laboratory manuals will have only one fungi exercise, and most of the time, it is mainly descriptive. As we developed this exercise we realized how much students benefit from working with the "shotgun" fungus "Pilobolus crystallinus" in an experimental setting. With this exercise students will learn about a basic reproductive cycle, enhance their lab skills, become familiar with data gathering, and write a lab report. In addition, students will use an experimental approach that prompts them to ask questions and make connections with the topic. This laboratory exercise is appropriate for advanced placement high school biology and first year college-level biology classes.