Gender inequality is strongly linked with poor performance in science and mathematics for female students in secondary schools owing to socio-economic and cultural issues as well as learning strategies. According to the Certificate of Secondary Education Examination results of 2021 in Tanzania, the failure rate for female students was higher than that for male students, and such failure in chemistry was associated with inadequate knowledge about the tested concepts, failure to identify the requirements of the respective questions and lack of adequate mathematical skills. Real practical sessions are integral parts of chemistry to enhance the learning environments for both female and male students. However, the real laboratory practices especially for chemistry are challenged with the costs of the construction of physical laboratory infrastructure and reagents, and the time-consuming and physical demands on personnel during real practical sessions. Thus, this study explores gender-based effects of the chemistry virtual laboratory against a paper-based approach towards a real chemistry practical in Tanzanian secondary schools. The results indicate that the combined exposures of virtual laboratory and paper-based practicals improve real practical performance for both female and male students. Moreover, the performance of real practical practices progressively improved for female students when they were first exposed to the virtual laboratory and then the paper-based practical. Such improvements can be associated with the virtual laboratory providing interactive learning environments which support female students to connect and engage.