The purpose of the present study was to examine the effects of the ePortfolio-based learning approach (ePBLA) on knowledge sharing and creation with 92 college students majoring in electrical engineering as the participants. Multivariate analysis of covariance (MANCOVA) with a covariance of pretest on knowledge sharing and creation was conducted for examining differences in knowledge sharing and creation between an experimental group (a total of 47 participants using ePBLA) and a control group (a total of 45 participants not using ePBLA or creating eportfolio). A t-test was also performed for examining the differences in knowledge sharing and creation of the experimental group before and after using ePBLA. The results showed that: a) the experimental group had significantly better knowledge sharing and creation than the control group; and b) the experimental group had significantly better knowledge sharing and creation after using ePBLA. The results implied that ePBLA facilitated knowledge sharing and creation. Furthermore, relationships between knowledge sharing and creation were also enhanced by the use of ePBLA.