This study examined how socio-demographic factors related to the levels of cultural and non-cultural prejudice among college students from Korea, China, and Japan. We used data collected from the Asian Value Survey. The main findings are as follows. First, Chinese students showed the lowest levels of cultural and non-cultural prejudice. Second, those who had higher cultural prejudice also showed higher levels of non-cultural prejudice. However, while Korean and Japanese students had significantly higher levels of cultural prejudice than non-cultural prejudice, a statistically significant difference was not found among Chinese students. Third, while levels of cultural prejudice were explained by the country of origin and income, those of non-cultural prejudice were explained by the country of origin, gender, and the size of the areas in which students resided during childhood. Future directions for research and multicultural education were suggested based on the findings.