Based on the job demands-resources model, the study developed and validated an instrument that measures physical education teachers' job demands-resources perception. Expert review established content validity with the average item rating of 3.6/5.0. Construct validity and reliability were determined with a teacher sample (n = 397). Exploratory factor analysis established a five-dimension construct structure matching the theoretical construct deliberated in the literature. The composite reliability scores for the five dimensions range from 0.68 to 0.83. Validity coefficients (intraclass correlational coefficients) are 0.69 for job resources items and 0.82 for job demands items. Inter-scale correlational coefficients range from -0.32 to 0.47. Confirmatory factor analysis confirmed the construct validity with high dimensional factor loadings (ranging from 0.47 to 0.84 for job resources scale and from 0.50 to 0.85 for job demands scale) and adequate model fit indexes (root mean square error of approximation = 0.06). The instrument provides a tool to measure physical education teachers' perception of their working environment.