ERIC Number: EJ1182196
Record Type: Journal
Publication Date: 2018-Jun
Pages: 5
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
Teaching Undergraduates LC-MS/MS Theory and Operation via Multiple Reaction Monitoring (MRM) Method Development
Betts, Thomas A.; Palkendo, Julie A.
Journal of Chemical Education, v95 n6 p1035-1039 Jun 2018
This laboratory experiment employs an innovative approach to introduce undergraduates to liquid chromatography-tandem mass spectrometry (LC-MS/MS) by developing a method based on multiple reaction monitoring (MRM). During this qualitative investigation, students operated a triple quadrupole mass spectrometer in the MS scan and product ion scan modes to determine precursor and product ions, respectively, for a vanillin, ethyl vanillin, and coumarin standard mixture. After identifying precursor [right arrow] product ion transitions, students conducted a 2 min liquid chromatography run in MRM mode. Students were astonished by the speed, selectivity, and sensitivity of the LC-MS/MS method compared to that of a liquid chromatograph with ultraviolet detection.
Descriptors: Undergraduate Students, Science Instruction, Science Laboratories, Laboratory Procedures, Measurement Equipment, Laboratory Experiments, Science Experiments, Spectroscopy, Hands on Science, Chemistry
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A