A highly instructive, wide-ranging laboratory project in which students study the effects of various parameters on the enzymatic activity of alcohol dehydrogenase has been adapted for the upper-division biochemistry and physical biochemistry laboratory. Our two main goals were to provide enhanced data analysis, featuring nonlinear regression, and also to give students experience in experimental design. Students use appropriate kinetic and thermodynamic equations to fit their data from Michaelis-Menten plots, enzyme activity pH profiles, inhibitor and denaturant concentration profiles, and temperature-dependence plots. Experiments at the end of this project are designed and implemented by student pairs, thus preparing them for independent research.