This study assessed the availability and use of ICT-based Instructional tools in selected medical colleges in Ogun State, Nigeria. This study adopted a descriptive survey research design. The population to be studied is medical lecturers (328), clinical instructors (42) and laboratory technologist (92) from Ben Carson Snr. Medical School, Babcock University, Obafemi Awolowo College of Health Sciences, and Olabisi Onabanjo University (OOU); total being 462. Simple random sampling technique was used to select 248 respondents for the study. This will represent the population to be studied. Data was collected with the use of titled questionnaires (ICT-bIPIMC) and a checklist during their faculty meetings/seminars, and the data collected were analysed using simple frequency and percentage techniques. It was revealed that Computer Aided Instruction (CAI), Social Media, Human Patient Simulation (HPS), PowerPoint Slides (Microsoft) [PP slides, and Medical videos (MV), Multimedia Classrooms (Audio Visual Centre), Projectors, E-Medical journals and animation clips] were the available ICT-based tools for Instructional tools in Ogun State medical colleges. Based on the available finding in this study, social media (45%), E-medical journals (40%), computer-aided instructions (39%), online educational forum (37%), and Internet connected laptops (34%), were the major ICT-based tools used at least twice daily. The study recommended that ICT-based instructional tools should be compulsory for instructional practices among lecturers, laboratory technologists and clinical instructors; also medical colleges should be motivated to use ICT-based tools as instructional tools through training and workshop on how to effectively use the available tools.