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ERIC Number: EJ1069010
Record Type: Journal
Publication Date: 2015-Jul
Pages: 6
Abstractor: As Provided
ISSN: ISSN-0021-9584
Student Collaboration in a Series of Integrated Experiments to Study Enzyme Reactor Modeling with Immobilized Cell-Based Invertase
Taipa, M. A^ngela; Azevedo, Ana M.; Grilo, Anto´nio L.; Couto, Pedro T.; Ferreira, Filipe A. G.; Fortuna, Ana R. M.; Pinto, Ine^s F.; Santos, Rafael M.; Santos, Susana B.
Journal of Chemical Education, v92 n7 p1238-1243 Jul 2015
An integrative laboratory study addressing fundamentals of enzyme catalysis and their application to reactors operation and modeling is presented. Invertase, a ß-fructofuranosidase that catalyses the hydrolysis of sucrose, is used as the model enzyme at optimal conditions (pH 4.5 and 45 °C). The experimental work involves 3 h of laboratory time for each student per week over four weeks. Students, organized in laboratory sessions of three groups with 3 or 4 students per group, work in a collaborative manner to obtain a set of replicates of initial reaction rate for different substrate concentrations, using both free and calcium alginate-immobilized "Saccharomyces bayanus" cells containing biologically active invertase. The results are shared by all of the groups for statistical data treatment, calculation of Michaelis-Menten kinetic parameters, and modeling of three types of classical reactors, a Continuous Stirred Tank Reactor (CSTR), a Plug Flow Reactor (PFR), and a Fluidized Bed Reactor (FBR), operating in continuous mode with immobilized invertase. For each reactor, the experimental conversion profile as a function of the feed flow rate is compared to predicted profiles based upon the kinetic parameters obtained.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: Portugal
Grant or Contract Numbers: N/A