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Wilkesman, Jeff; Castro, Diana; Contreras, Lellys M.; Kurz, Liliana – Biochemistry and Molecular Biology Education, 2014
This laboratory exercise presents a novel way to introduce undergraduate students to the specific detection of enzymatic activity by electrophoresis. First, students prepare a crude peroxidase extract and then analyze the homogenate via electrophoresis. Zymography, that is, a SDS-PAGE method to detect enzyme activity, is used to specifically…
Descriptors: Science Laboratories, College Science, Undergraduate Students, Molecular Structure
Stourman, Nina; Moore, Jennifer – Biochemistry and Molecular Biology Education, 2018
Lactase is the enzyme responsible for the digestion of the disaccharide lactose, and deficiency in this enzyme causes the prevalent medical condition lactose intolerance. Management of lactose intolerance can be achieved through the administration of lactase supplements. Lactase is an appropriate platform for advanced enzymatic study because its…
Descriptors: Biochemistry, College Science, Science Laboratories, Inquiry
Liuzzi, G. M.; Mastroianni, C. M.; Latronico, T.; Mengoni, F.; Fasano, A.; Lichtner, M.; Vullo, V.; Riccio, P. – Brain, 2004
The introduction of potent antiretroviral drugs for the treatment of patients with human immunodeficiency virus (HIV) infection has dramatically reduced the prevalence of HIV-associated neurological disorders. Such diseases can be mediated by proteolytic enzymes, i.e. matrix metalloproteinases (MMPs) and, in particular gelatinases, released from…
Descriptors: Sexually Transmitted Diseases, Drug Therapy, Outcomes of Treatment, Dementia