Quality of working life has been defined as the part of overall quality of life that is influenced by work. We developed a mediation model where home-work interface, job and career satisfaction, control at work, and working conditions are considered to be positively related to employee commitment and to the absence of stress at work. These two variables were ultimately related to general well-being. We considered possible differences between workers with different contract types (permanent vs. temporary), as well as the roles of tenure and average position of the University in the UK rankings. Quantitative survey data from 510 academics and researchers working in 8 British Universities were analysed via a multi-group analysis with structural equation modelling. Our model presented a good fit, and a bootstrapping analysis confirmed the significance of indirect effects. Significant differences were found between permanent and temporary workers and striking results were found regarding tenure.