The Holistic Wellness Assessment (HWA) is a relatively new assessment instrument based on an emergent transdisciplinary model of wellness. This study validated the factor structure identified via exploratory factor analysis (EFA), assessed test-retest reliability, and investigated concurrent validity of the HWA in three separate samples. The hypothesized eight-factor structure was validated via confirmatory factor analysis (CFA), individually for each factor and overall in a multifactor analysis. Test-retest reliability estimates over a 1- to 3-week interval were appropriate for this assessment type. Concurrent validity estimates indicated that the HWA measures were similar, but not redundant, to wellness constructs found in other wellness instruments, specifically the TestWell® and Wellness Evaluation of Lifestyle, Version S (WEL-S). As young adults are exposed to a broader base of wellness in educational and related contexts, the use of the wellness assessments such as the HWA can identify areas of personal need for balance and healthy choice making.