Purpose: The purpose of this paper is to show that HIV/AIDS-related stigma has persisted world-wide for decades. However, studies on the linkage between stigmatizing attitudes towards people living with HIV/AIDS (PLWHA) and misconceptions about HIV transmission routes in the general population, especially among youth in China, are sparse--a gap this study is intended to fill. Design/methodology/approach: Cross-sectional data from 1,839 students from 19 colleges were collected by trained interviewers using a structured questionnaire in Jiangsu province of China. Findings: This study reveals that there is a high proportion of college students having both stigmatizing attitudes toward PLWHA and misconceptions about HIV/AIDS transmission routes. Multilevel logistic regression analysis results show that having stigmatizing attitudes towards PLWHA is positively associated with having misconceptions about HIV transmission routes. Participants with high misconception scores were more likely to possess stigmatizing attitudes towards PLWHA. Originality/value: To reduce stigmatizing attitudes towards PLWHA, HIV/AIDS education should be strengthened among the general population, especially among youth. (Contains 5 tables.)