In order to better understand the effects of student-life stress on Education and Health Service majors (n = 195) at a private, religious, Midwestern university in the USA, we assessed student perception of overall stress level and physical stress level using the Student-life Stress Inventory. The targeted sample consisted of students with declared majors that lead to careers involving interaction with vulnerable populations (i.e., children, individuals with disabilities, patients). Women in this study experienced a significantly higher (F = 7.092 [1195], p = 0.008) overall stress level than their male counterparts. When the physical stress reaction scores were isolated, women still experienced significantly higher stress levels (F = 7.758 [1195], p = 0.01). Regardless of gender, seniors and graduate students had an overall stress difference that was 11 points below that of freshman students in overall stress scores. There was a significant difference in the overall stress score (F = 6.484 [2181], p = 0.001) between students who took 18 or more credit hours and those who took less. Students who worked more than four hours per week had a significantly higher overall stress level. Students in the age category 23+ had significantly less physical stress. There was a significant difference between students who prayed at least daily and students who never pray (F = 1.114 [3188], p = 0.03). This study provides a valuable insight into the characteristics of individuals more likely to have difficulties adjusting to the demands of college life and the inherent stress of a profession in Education or Health Service. (Contains 1 table and 1 note.)