In the nursing profession, post-graduate in-service trainings are scheduled to increase efficiency and improve the quality of service. There are barriers to participation in training programs due to some reasons such as the intense work pace, incompatible shifts, a lack of personnel, the training program is not being at the knowledge and skill level of the personnel to be trained, the poor physical environment of the training environment. The purpose of this methodological study is to develop mobile training material to provide nurses working in university hospital with professional literature and also, to evaluate its visual design, reliability and the quality of information in the developed training material. The information quality, reliability, readability and visual design of the mobile training material developed for use on Android and IOS smartphones was assessed by 10 experts, twice, by means of the Discern Measurement Questionnaire and Visual Design Control Scale. After the first expert evaluation, the content was updated and a second evaluation completed. Content validity index (CVI) analyzes were performed. The Discern Measurement Questionnaire's total score for the training material configured for smartphones was calculated at 70.80 ±2.57 out of 75. CVI analysis results of the Discern Measurement Questionnaire and Visual Design Control Scale scales of the training material were found to be 0.75 and 0.78, respectively. According to Gagne's events of instructions, the information quality and the reliability of the training material configured for smartphones is high and this training material is validated.