Indiana recently passed legislation requiring teacher preparation programs to educate future teachers on how to identify and refer struggling readers, including students with learning needs related to dyslexia. The purpose of this study was to examine whether a university course that covers content in response to legislation was effective in improving preservice teachers' knowledge about the topic of dyslexia. Participants included 62 undergraduate students enrolled in general education teacher preparation programs. One group of 23 participants had taken a required course with content regarding reading challenges and targeted instruction; the remaining 39 participants had not yet taken the required course. Preservice teachers who took the course had more accurate knowledge about dyslexia than those who had not taken the course. There were no differences between groups for confidence in ability to help students struggling with dyslexia. Additional implications regarding legislation and teacher preparation will be included in the discussion.