Plagiarism is a growing concern in Healthcare Sciences in the current digital era. Plagiarism threatens institutions' integrity and academic reputation; therefore, it is essential to understand all contributing factors to eradicate unethical practices effectively. The study aimed to establish the knowledge, attitudes, and practices of plagiarism among Healthcare Science students at a selected higher education institution in Gauteng. A cross-sectional study was used, with a total population of 803 second to fourth-year students from the School of Healthcare Sciences, and 83 students completing the survey. Ethical clearance and approval (617/2021) were obtained from the ethics and research committee of a selected higher educational institution in Gauteng. An adapted Likert scale self-reported questionnaire was sent via a Google form link to the teaching platform with the consent form embedded. The statistical Package for Social Science was used to analyse demographic data descriptively, and Spearman's rho test determined the correlation between study variables. Most students were female (94.0%) and non-native English speakers (71.1%). The majority (83.1%) of the students reported understanding the instances of plagiarism; however, approximately one-third (36.1%) were unable to identify specific instances correctly. Just over half (56.6%) of the students felt self-plagiarism should not be punishable. The correlation between the knowledge of plagiarism and negative attitudes towards plagiarism was found to be statistically significant (p=0.009). The results demonstrated low practice of plagiarism, possibly due to a lack of self-awareness. Therefore, continuous in-depth education on plagiarism and improved policies should be fostered to promote the integrity of future healthcare professionals.