We describe opinions on medical use of "Cannabis sativa L." under conditions of Slovakia (n = 717). Personal experience with marijuana was detected in 77.42% (n = 553) in age categories younger than 20 years (n = 96) and in 77.06% (n = 457) of adults. Almost 86% of respondents (n = 618) agreed with legal use of marijuana for medical purposes. Furthermore, respondents' views on legal usage of marijuana for medical purposes could be affected by availability of information (r = 0.12) and personal experience (r = 0.23). Negative impact of substance abuse in younger age-groups was recognized as threat (r = 0.47), but knowledge about harmful effect of marijuana use did not affect personal opinion for decriminalization (r = 0.38).