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Filgueiras, Matheus Fernandes; Borges, Endler Marcel – Journal of Chemical Education, 2022
Students quantified the protein content in beer, milk, powdered milk, and whey protein using the Bradford assay. The assay was carried out using absorbance measured at 595 nm (standard method) and 96-well-plate images (proposed method). They built analytical curves using bovine serum albumin (BSA) and casein and determined that protein type…
Descriptors: Chemistry, Color, Visual Aids, Science Instruction
Filgueiras, Matheus Fernandes; de Jesus, Paulo Cesar; Borges, Endler Marcel – Journal of Chemical Education, 2021
The Griess assay is widely used by regulation agencies as an official method for nitrite quantification in water and food samples. In Brazil, the official method, which has been used to determine nitrite in food, was described by Instituto Adolfo Lutz (283/IV) in 1984. It uses 8 mL of reactants and provides 50 mL (reactants plus sample) of waste…
Descriptors: Science Instruction, Water, Food, Science Experiments
Sidou, Lais Feltrin; Borges, Endler Marcel – Journal of Chemical Education, 2020
Principal component analysis (PCA) is one of the most important and powerful methods in chemometrics as well as in a wealth of other areas. Running a PCA results in two main elements, the score plot and the loading plot; the score plot provides the location of the samples, and the loading plot indicates correlations among variables, the trends in…
Descriptors: Factor Analysis, Chemistry, Science Instruction, Teaching Methods
da Silva, Rodrigo Sens; Borges, Endler Marcel – Journal of Chemical Education, 2019
Here, students determine aspirin (acetylsalicylic acid) mass in pharmaceutical tablets using a colorimetric method. Aspirin, salicylate, and salicylic acid do not absorb visible light. Thus, in alkaline medium, acetylsalicylic acid was hydrolyzed to salicylate; then, it was reacted with an acidic Fe(III) solution, and a violet complex was formed.…
Descriptors: Spreadsheets, Drug Therapy, Light, Chemistry
Ledesma, Claudia Martins; Krepsky, Larissa Mascarenhas; Borges, Endler Marcel – Journal of Chemical Education, 2019
Here, students determine the total phenolic content in beers using the Folin-Ciocalteu assay. The Folin-Ciocalteu reagent is a yellow complex, in an alkaline medium; it reacts with phenols and non-phenolic reducing substances to form a blue complex. Quantitative analysis was carried out using absorbance measured at 765 nm (standard method) and…
Descriptors: Science Instruction, Science Experiments, Chemistry, College Science