The aim of this study is to compare some physical parameters of professional and regional amateur footballers according to their positions. Totally 100 volunteer football players playing in the professional and regional amateur league participated in the study. Anthropometric measurements (height, body weight, body fat ratio) were performed first in evaluating the physical parameters of football players. Second, anaerobic capacity (flexibility, vertical jumping, speed) and third one is aerobic capacity (yo-yo IR1) measurements were completed. Kolmogorov Smirnow test was used for normality testing of the data used in the study. Thus, it is understood that the data show normal distribution, one-way analysis of variance (one way ANOVA), which is one of the parametric tests, was also used in cases where more than two groups were compared. The statistical analysis used in the study was performed at 0.005 error levels in 95% confidence interval. SPSS 22.0 package program is applied for statistical analysis of the data. As a result, it is observed that the football players participating in the study have statistically difference between the flexibility, vertical jumping and 30 m speed values according to the leagues, and height, body weight and body fat percentage values according to the positions (p < 0.05). Having the flexibility, vertical jumping and 30 m speed works to improve the parameters of the players with meaningful differences can be made to the players who play in all leagues and positions can contribute to the increase in performance.