Background: Computer-assisted tools are rarely adopted for dental education in China. In China, 3D digital technology, such as Virtual Reality Systems, are often rejected in the dental field due to prohibitive pricing. There is also a reluctance to move away from traditional patterns of dental education. Objective: The current study is one of a series of research programs designed to investigate the effects of introducing a software program, named the Dental 3D Multimedia System (D3DM), into the education of a group of junior dental students in their preclinical practice. It was theorized that using 3D technology would improve certain aspects of subjects' performance, thus lending support to the idea that electronic media is a useful & valuable means for dental education in China. Methods: A paired-sample contrast study between two groups of dental students was conducted. One group received their training program in the traditional way, unassisted by 3D technology. The second group received their training program in the traditional manner, but also used the D3DM to supplement their education. Data indicating the study efficiency of the two groups were gathered and analyzed. The results between the two groups were then compared. Results: Statistical differences were detected on several points between the two groups. These differences indicated that the D3DM-assisted group worked faster, and no worse than the traditional group during the training in labs. The D3DM-assisted group also spent less time interacting with faculty members and seeking feedback during practice. Finally, the results also show that the D3DM-assisted group achieved higher scores in theory exams. Conclusion: The results indicate that the usage of 3D multimedia PC software had a positive impact on several aspects of subjects' performance. At present, no significant drawbacks from applying the software have been identified.