Objective: The systematic review was used to evaluate the relationship between fundamental movement skills (FMS) and physical activity (PA) in preschoolers. Data sources: PubMed, Science Direct, Web of Science, CNKI, and Wanfang databases were searched for terms related to preschoolers' FMS and PA from the establishment to July, 2022. Results: 21 cross-sectional studies and 5 longitudinal studies with 6561 preschoolers were included. There was a significant positive association between FMS and moderate-to-vigorous physical activity (MVPA) (r = 0.25, 95%CI 0.22[approximately]0.27) and total physical activity (TPA) (r = 0.23, 95%CI 0.19[approximately]0.27). The locomotor and object control skills were significant positive association with both MVPA and TPA. Conclusion: There is a significant positive association between FMS and MVPA as well as between FMS and TPA, indicating the movement proficiency is associated with increased PA and vice versa.