This paper aims to provide public health organisations involved in chronic disease prevention with conceptual and practical guidance for developing contextually sensitive knowledge-to-action (KTA) strategies. Methods involve an analysis of 13 relevant conceptual KTA frameworks, and a review of three case examples of organisations with active KTA agendas. From this analysis, this paper identifies and discusses four key principles for enhancing organisational KTA strategy: (1) align knowledge production and action; (2) foster connections among relevant stakeholders; (3) understand and work with key contextual factors; and (4) consider a diverse yet coherent set of KTA activities.