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ERIC Number: EJ1088168
Record Type: Journal
Publication Date: 2016-Jan
Pages: 4
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
Spectrophotometric Determination of 6-Propyl-2-Thiouracil in Pharmaceutical Formulations Based on Prussian Blue Complex Formation: An Undergraduate Instrumental Analysis Laboratory Experiment
Zakrzewski, Robert; Skowron, Monika; Ciesielski, Witold; Rembisz, Zaneta
Journal of Chemical Education, v93 n1 p182-185 Jan 2016
The laboratory experiment challenges students to determine 6-propyl-2-thiouracil (PTU) based on Prussian blue complex formation. Prussian blue is formed by ferricyanide and Fe(II) ions which are generated in situ from Fe(III) ions reduced by PTU. The absorbance of this product was measured at a wavelength of 840 nm, after a reaction time of 30 min. The range of determination was 0.34-3.4 µg mL[superscript -1] of PTU per sample. The method was successfully used in an upper-division instrumental analysis laboratory course to determine the concentration of PTU in commercially available pharmaceuticals. Additionally, students in introductory or high school chemistry classes by conducting this experiment will gain a greater understanding of the chemical analysis process, redox reactions, and the Beer-Lambert law.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education; High Schools; Secondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A