ERIC Number: EJ920085
Record Type: Journal
Publication Date: 2010-Sep
Pages: 2
Abstractor: As Provided
ISBN: N/A
ISSN: ISSN-0021-9584
EISSN: N/A
An Enzyme Kinetics Experiment for the Undergraduate Organic Chemistry Laboratory
Olsen, Robert J.; Olsen, Julie A.; Giles, Greta A.
Journal of Chemical Education, v87 n9 p956-957 Sep 2010
An experiment using [superscript 1]H NMR spectroscopy to observe the kinetics of the acylase 1-catalyzed hydrolysis of "N"-acetyl-DL-methionine has been developed for the organic laboratory. The L-enantiomer of the reactant is hydrolyzed completely in less than 2 h, and [superscript 1]H NMR spectroscopic data from a single sample can be worked up to generate Michaelis-Menten and Lineweaver-Burke kinetic plots. The experiment introduces organic chemistry students to the use of a [superscript 1]H NMR spectrometer, serves as an introduction to enzyme kinetics, and illustrates the stereospecificity of enzyme catalysis. (Contains 4 figures and 3 notes.)
Descriptors: Kinetics, Organic Chemistry, Science Laboratories, Biochemistry, Science Instruction, Science Experiments, College Science, Scientific Principles, Spectroscopy, Undergraduate Study
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A