Substrate selectivity of "Candida rugosa" lipase was tested using "p"-nitrophenyl esters of increasing chain length (C[subscript 1], C[subscript 7], C[subscript 15]) using the high-throughput screening method. A fast and easy 96-well microplate bioassay was developed to help students learn and practice biotechnological specificity screen. The kinetic constants V[subscript max] and K[subscript m] of each substrate were compared to indicate the lipase activity and selectivity to different substrates. In this experiment, students were exposed to the core concepts of Michaelis-Menten kinetic analysis and learned the principles and techniques of studying substrate selectivity, including the use of a microplate reader and the associated software, the layout of bioassays, and the multichannel-repeater pipetting. (Contains 1 table, 1 figure and 1 scheme.)