TransAsia Airway is one of the four domestic airlines in Taiwan. Taiwan has 13 domestic airports with the longest distance between two airports being about 400 kilometers. The domestic airline market is highly competitive. TransAsia decided to apply e-learning within its organization to reduce training expenses and improve service quality. This study describes the strategy and methodology used by TransAsia when applying e-learning for regular training, and considers the management changes made by TransAsia when reconstructing its organization behavior and learning culture using e-learning. This study also uses the Krikpatrick model to assess the effect of e-learning within a firm. The TransAsia case is used to provide a reference for the future development of the Taiwanese airline industry. (Contains 2 figures and 12 tables.)