Currently very few Thai Immunology e-Books are available online. The authors created an online e-Book titled, "Antigen and Antibody Reaction for Diagnosis of Diseases" and used a quasi experimental research design to assess the effectiveness of its implementation in terms of knowledge gained, written exam scores and student satisfaction. Both the experimental and control groups exhibited higher mean scores of between the post- and pretest at p less than 0.001, as calculated by paired t-test. The two groups' Immunology mean scores were not significantly different. All participants passed the Immunology examination. It is hoped that further improvements to the e-Book and consistent Internet access will result in statistically significant differences for e-Book users in the future.