Background: The authors have conducted a number of research projects into the use of ICT in science teaching and most recently have collaborated with five European partners in teacher education to develop resources to assist teacher trainers in delivering courses for the professional development of science teachers. Purpose: 1. To describe the main aspects of pedagogy which are relevant to the use of ICT tools which serve practical science teaching. 2. To discuss approaches to teacher education which aim to emphasise the pedagogical aspects of using those ICT tools. Sources of evidence: 1. A review of the research literature on the effectiveness of using ICT in education with a particular focus on pedagogical knowledge and its interaction with associated technical knowledge. 2. Authors' experience as teacher trainers and as researchers in methods of employing ICT in science education. 3. Studies conducted by partners in the "ICT for Innovative Science Teachers" Project and training materials developed by the project. Main argument: Starting from the premise that it is the pedagogical actions of the teacher which determine successful learning outcomes of using ICT in science lessons, the paper describes the main components of pedagogical knowledge and understanding required by teachers. It examines the role of an understanding of affordances in helping teachers to deploy software tools appropriately and defines some of the skills for exploiting them to benefit learning. Innovation is successful when ICT activities are incorporated in ways that complement non-ICT activities and serve science learning objectives. When teachers are alert to adapt their pedagogical skills, they evolve new ways of working and interacting with students. Training courses need to provide means of helping teachers to examine the professional beliefs which underpin their pedagogical approaches. This is most effectively achieved when a course blends personal hands-on experience with discourse with other professionals and when there is iteration between the training experience with activity in the classroom. Conclusions: The most significant products of professional development are the integration of ICT in the curriculum and a change in a teacher's pedagogy towards teaching approaches which empower students to work more independently and reflectively.