A survey measure was developed to assess high school students' experience, knowledge, and self-confidence related to health care careers. In the fall and spring of one school year, the measure was administered to a diverse sample of 2,309 students participating in career academies focused on the health care industry. Confirmatory factor analyses (CFA) supported the reliability and validity of the measure. Using latent difference score modeling, the findings showed that the students exhibited moderate gains in experience (ES = 0.38) and knowledge (ES = 0.37) across the school year, suggesting that the career academies positively impacted their health care career readiness. The students exhibited very little growth in their self-confidence related to health care careers during the same period (ES = 0.05).