The purpose of this study was to examine the effect of a 16-week yoga program on blood pressure (BP) in healthy college students. Twenty-five students (Mage = 28.24, SD = 10.64) participated in yoga class twice per week for 16 weeks. Thirty-one students (Mage = 28.77, SD = 7.23) attended a lecture (control condition) at approximately the same time as yoga. Resting systolic blood pressure (SBP) and diastolic blood pressure (DBP) were assessed at the beginning and end of the 16-week semester. Repeated measures ANCOVAs with pre-study BP as the covariate were used to test group BP differences across time (pre- to post-study). The SBP ANCOVA indicated yoga had a moderate statistically significant greater effect on lowering SBP compared to the control condition (p = 0.03, ES = 0.61). The DBP ANCOVA revealed that yoga produced a small statistically nonsignificant greater effect on lowering DBP compared to the control condition (p = 0.22, ES = 0.24). After the additional demographic variables were considered, regression analyses showed that the effect of yoga on lowering SBP compared to the control remained significant (p = 0.04), and the effect of yoga on lowering DBP compared to the control remained nonsignificant (p = 0.32). The results support regular yoga practice as a method of lowering SBP in healthy college students.