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Peer reviewed Peer reviewed
ERIC Number: EJ338061
Record Type: CIJE
Publication Date: 1986
Pages: N/A
Abstractor: N/A
ISBN: N/A
ISSN: N/A
EISSN: N/A
How to Illustrate Ligand-Protein Binding in a Class Experiment: An Elementary Fluorescent Assay.
Marty, Alain; And Others
Journal of Chemical Education, v63 n4 p365-66 Apr 1986
Describes an experiment (taking approximately five hours) which illustrates the binding of a small molecule to a protein. By using an appropriate fluorescent ligand and a given protein, the fluorescent probe technique is applied to measure the number of bonding sites, and number of site classes, and their association constants. (JN)
Descriptors: Chemical Bonding, Chemistry, College Science, Higher Education, Laboratory Procedures, Science Education, Science Experiments
Publication Type: Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: Teachers; Practitioners
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A