The prevalence and contributing factors of workplace bullying (WPB) are unknown among school nurses (SNs) in kindergarten to 12th grade programs. The aim of this cross-sectional study was to examine individual and organizational characteristics of WPB in a sample of SNs in Virginia. Based on the Short-Negative Acts Questionnaire, 40% of nurses did not experience bullying behavior, 34.8% of nurses faced occasional bullying (now and then or monthly), and 25.3% of nurses were frequently bullied (weekly or daily). Backward stepwise regression demonstrated the predictor variables of being non-White, a licensed practical nurse, or not involved in student individual education plans were significantly associated with being bullied. Administrators/supervisors need to be aware of the existence of WPB.