A multistep synthesis of 10-ethyl flavin was developed as an organic chemistry laboratory experiment for upper-division undergraduate students. Students synthesize 10-ethyl flavin as a bright yellow solid via a five-step sequence. The experiment introduces students to various hands-on experimental organic synthetic techniques, such as column chromatography, thin layer chromatography (TLC), extraction techniques, and characterizing intermediates and final products by IR and NMR spectroscopy. It also provides an opportunity for students to review important topics such as nucleophilic substitution reactions, hydrolysis of amides, condensation reactions, and reduction of aromatic nitro groups to amines.