Within the higher education sector in Australia regionality is not well defined, which is a significant issue for regional universities, given the opportunities for development and growth stemming from the Australian Government's focus on regional higher education. This paper contends that if regional universities are to operate successfully in an increasingly competitive sector, they need to centre their operations around a clear definition of regionality. Based on a review of definitions of regionality in international higher education contexts, and an analysis of how regionality is defined outside of higher education, this paper provides a definition of regionality for Australian higher education. Whilst acknowledging the differences in potential contexts and applications, this definition seeks to provide a basis upon which regional universities in Australia can determine the ways in which they may create a strong identity, achieve the Australian Government's objectives for regional higher education, and reap the associated benefits.