The influence of body hydration and vocal acoustics was investigated in this study. Effects of two levels of hydration on objective measures of vocal acoustics were explored. In an attempt to reduce variability in the degree of systemic hydration and to induce a state of systemic dehydration, participants were instructed to refrain from ingestion of food and liquids for 12 h prior to testing. Following the pretest (i.e., dehydration condition), each participant in the experimental group ingested 1 L of water within 20 min (i.e., rehydration condition). Vocal acoustics were collected at pretest and posttest by using vowel samples, which were then analyzed. Results related to the impact of the hydration condition on shimmer vocal acoustics averaging the 5 vowels for each subject in the analysis were found to have statistical significance, leading to the conclusion that the hydration condition had a positive impact on shimmer vocal acoustics. (Contains 5 tables.)