Listening comprehension in English, as one of the most fundamental skills, has an essential role in the process of learning English. Mokken scale analysis (MSA) is a probabilistic-nonparametric approach to item response theory (IRT) which determines the one-dimensionality and scalability of test. Mokken scaling techniques are a useful tool for researchers who wish to construct unidimensional tests or use questionnaires that comprise multiple binary or polytomous items. The current study utilized the MSA to examine one-dimensionality and scalability of TEOFL iBT listening test, administered on 400 EFL university students in the Iranian context. The results illustrated no main concerns in terms of item scalability. Monoton homogeneity model (MHM) fitted all the items of the test very well as measured by the scalability coefficients and restscore groups method. Considering the invariant item ordering (IIO), it was concluded that the ordering of items according to their mean is invariant across examinees, although HT was small. Dimensionality analysis results using the automated item selection procedure (AISP) depicted that the test is unidimensional-confirming evidence of the validity of the test in measuring a single ability dimension. The empirical application depicted a potential and feasible approach, whereby MSA could be used as a valuable method for exploring the behavior of scaled items in response to varying levels of a latent trait in education research.