Numerical estimation has been used to study how children mentally represent numbers for many years (e.g., Siegler & Opfer, 2003). However, these studies have always presented children with positive numbers and positive number lines. Children's mental representation of negative numbers has never been addressed. The present study tested children in the 2nd, 4th, and 6th grades to assess their mental representations of both positive and negative numbers using a standard numerical estimation task. We replicated the shift from a logarithmic to linear representation for positive numbers (0-1,000 scale) in that 2nd graders represented positive numbers logarithmically, but 4th and 6th graders represented the numbers linearly. Furthermore, children's representation of negative numbers paralleled their representations of positive numbers and showed the same shift from a logarithmic representation at Grade 2 to linear representations at Grades 4 and 6. This is the first study to provide data on children's representation of negative numbers, and the implications of these findings are discussed.