The purpose of this study was to assess the effects of three choral configurations on a soprano section's sound. The first configuration resembled a choir section without an assigned standing position, the second configuration grouped singers by timbre, and the third used acoustic-compatibility placement. Three conductors configured a university soprano section (N = 13) who were audio-recorded singing in each configuration and answered questions about their perceptions. Audio recordings were analyzed acoustically using long-term average spectra and perceptually through pitch analysis and listener perceptions. Results indicated that participants sang with significantly increased spectral energy in the acoustic-compatibility configuration (p < 0.001), and both singer and listener participants preferred intentional standing configurations over the random standing configuration. Findings from this study suggest that choral directors can use intentional configurations in conjunction with 2 ft intersinger spacing to improve singer comfort and overall sound.