There is growing recognition that after-school programs (ASPs) provide opportunities for positive youth development. Many ASPs focus on behavior and socio-emotional challenges, provide evidence-based interventions to improve homework completion and academic skills, and offer physical activities and nutritious foods. Generally speaking, ASPs offer a critical service to families by providing safe environments for students during ''risky'' after-school hours. These programs have an opportunity to reap many benefits by utilizing evidence-based education initiatives, such as positive behavior interventions and supports (PBIS), to promote a positive environment, support participant and staff behavior, and enhance outcomes. In support of extending PBIS into ASPs, this paper includes an overview of PBIS, specifies its relevance to ASPs, outlines initial research support, and reviews considerations related to preparation, implementation, and evaluation of PBIS in ASPs. Considerations for embedding PBIS into ASPs during preparation, implementation, and evaluation are provided.