The research aims to know the effect of Active Learning on the concept of mastery of sains learning by fifth grade students at primary school. The method that is used in this research is a quasi-experimental research. The aspect being measured in this research was comparing two learning groups. Experiment group was exposed to Active Learning Model and control group to conventional learning model. The data population of this research was all fifth grade students (28 students) at Primary School Soropaten 2 district Klaten in academic year of 2017/2018. The population was then divided into two classes. 14 students were in the experiment class, and 14 students were in the control class. The data sampling of research conducted with cluster random sampling. Data collection techniques included tests and observations. Data analysis was done by using a t-test and descriptive statistics. The results showed that learning with Active Learning Model influences the concept mastery of sains learning. The result learning by fifth-grade students Primary School showed 2.990 with the significance level at less than 0.05 i.e., 0.006.