The purpose of this study was to examine the effects of gender and academic achievement scores on pre-service science teachers' self-reported use of motivational strategies and learning strategies. The present study also investigated the relationship between motivational strategies and learning strategies. Data were collected by using Turkish version of Motivated Strategies for Learning Questionnaire (MSLQ) and personal information sheet during 2010-2011 spring semester. MSLQ which was originally developed as a self-report questionnaire, comprised of two main dimensions as motivation and learning strategies including 81 items with a 7 point-Likert scale format. The present study was designed as cross-sectional. A total 104 pre-service science teachers voluntarily participated in the study. Results revealed that pre-service science teachers who had higher academic achievement scores in physics, also had higher self-efficacy for learning beliefs. It was also found that there were no statistically significant mean differences among motivational beliefs, self-regulated learning variables with respect to gender. Lastly, the subdimensions in the motivational and learning strategies sections were found to be positively correlated as specified by the theory.