The aim of this research is to reveal the accumulation of musical culture of preschool teachers and to get their viewpoints about music culture. The sub-objectives of the research are consisted of finding answers to "To what extent pre-school teachers support the music activities in the city where they work", "how often they participate in these music activities", "what it means to have music culture accumulation in their individual life and educational life". This research was conducted with a survey model and the sample of the research constitutes 221 pre-school teachers studying in Afyonkarahisar city center while its sample is consisted of 55 preschool teachers. The questionnaire was applied to the pre-school teachers who were selected as the sampling in terms of determining their views about music culture and accumulation of their music culture. The data gained from this study was analyzed by a package program named as IBM SPSS statistics version 20. While investigating the relationships among the nominal variable groups, chi-square analysis was performed. On the condition that the expected values in the entries of the 2*2 tables did not have the sufficient volume, Fisher's exact test was used, while in the RxC tables Pearson chi-square analysis was performed with the help of Monte Carlo Simulation. Thus, the existing situation has been determined and what it should be has been found out. This music culture survey involves questions such as "How the pre-school teachers' musical education in the undergraduate study period affects their musical cultures", "What kind of music they listen", "At what level they follow the music activities" and "How musical cultures influence their professional areas and their general life". This study sheds light on the fact that it is necessary to propose and improve the preschool education program in pre-school teacher education period in terms of music culture and music education, and has fundamental aspects in relation to being a source for the future studies on music culture. [This study has been presented as an oral representation paper at the 3rd International Conference on Lifelong Education and Leadership in Polytechnic University of Porto, Portugal, 12-14 September, 2017.]