This study assessed the prevalence of health behaviors among non-academic staff at a Midwestern U.S. university. A sample of 627 women and 237 men completed a web survey for the study. Most of the health behaviors showed no sex difference. Health behaviors of drinking, smoking, and irregular breakfast eating were significantly associated with amount of education. Logistic regression analysis indicated that sleeping less than 5.5 hours (odds ratio: 8.44; 95% CI: 3.55, 20.05), coffee drinking (odds ratio: 1.63; 95% CI: 1.12, 2.37), currently smoking (odds ratio: 3.74; 95% CI: 2.25, 6.21), infrequent exercise (odds ratio: 2.01; 95% CI: 1.4, 2.90), and regular soda consumption (odds ratio: 2.13; 95% CI: 1.53, 2.95), were associated with irregular breakfast eating. Amount of education was associated with health-compromising behaviors and needs to be considered during health promotion programs. (Contains 3 tables.)