The use of education applications in language learning is rapidly increasing. This research aims to analyse the tonal perception ability of Chinese students using the Thai Tone Application with minimal pairs approach. The participants were divided into two groups, an experimental group (ones who studied in the class and used the application) and a control group (ones who studied in the class without using the application), of 20 students each. One-syllable words bearing low tone and falling tone were used to examine the participants' tonal perception ability. The findings revealed that the experimental group significantly outperformed the control group in terms of differentiating between low tone and falling tone. The result of the current study further indicates the use of the mobile-assisted language learning application since supplemental material may effectively enhance Thai tone perception or Thai tone learning outcomes in some Chinese students. The significance of the minimal pair approach is also discussed.